Affiliation:
1. Department of Microbiology, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden
Abstract
ABSTRACT
The gene
fnz
from
Streptococcus equi
subspecies
zooepidemicus
encodes a cell surface protein that binds fibronectin (Fn). Fifty tested isolates of
S. equi
subspecies
equi
all contain DNA sequences with similarity to
fnz
. This work describes the cloning and sequencing of a gene, designated
fne
, with similarity to
fnz
from two
S. equi
subspecies
equi
isolates. The DNA sequences were found to be identical in the two strains, and sequence comparison of the
fne
and
fnz
genes revealed only minor differences. However, one base deletion was found in the middle of the
fne
gene and eight base pairs downstream of the altered reading frame there is a stop codon. An Fn-binding protein was purified from the growth medium of a subspecies
equi
culture. Determination of the NH
2
-terminal amino acid sequence and molecular mass, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed that the purified protein is the gene product of the 5′-terminal half of
fne
. Fn-binding activity has earlier only been found in the COOH-terminal half of FNZ. By the use of a purified recombinant protein containing the NH
2
half of FNZ, we provide here evidence that this half of the protein also harbors an Fn-binding domain.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
47 articles.
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