Production of Recombinant Proteins in the lon -Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone-Reference-Cited by-同舟云学术

Production of Recombinant Proteins in the lon -Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone

Published:2009-06 Issue:11 Volume:75 Page:3803-3807
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ratelade Julien¹,Miot Marie-Caroline²,Johnson Emmett³,Betton Jean-Michel²,Mazodier Philippe⁴,Benaroudj Nadia⁴

Affiliation:

1. Unité de Nephropathies Héréditaires et Rein en Développement, INSERM U574, Hôpital Necker-Enfants Malades, 149 Rue de Sèvres, 75743 Paris Cedex 15, France

2. Institut Pasteur, Unité de Biochimie Structurale, CNRS URA 2185, F-75015 Paris, France

3. Department of Microbiology and Immunology, Tulane Medical School, 1430 Tulane Avenue, New Orleans, Louisiana 70112

4. Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, CNRS URA 2172, F-75015 Paris, France

Abstract

ABSTRACT To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) Δ dnaK strain of Escherichia coli . Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensability for protein production in BL21(DE3), which is void of Lon protease, key to eliminating unfolded proteins.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00255-09

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