Formations of Extracellular Isoamylase and Intracellular α-Glucosidase and Amylase(s) by Pseudomonas SB15 and a Mutant Strain

Author:

Sugimoto Toshiyuki1,Amemura Akinori1,Harada Tokuya1

Affiliation:

1. The Institute of Scientific and Industrial Research, Osaka University, Suita-shi, Osaka, Japan

Abstract

Pseudomonas SB15, which produces extracellular isoamylase, was found to produce intracellular α-glucosidase and amylase(s) when grown on maltose. A mutant strain (MS1) derived from it, which formed isoamylase constitutively, also produced these intracellular enzymes constitutively. The activities of the enzymes produced in the mutant strain were much greater than those induced in the parent strain.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference10 articles.

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3. Structural analysis of amylopectin using Pseudomonas isoamylase;Akai H.;Biochim. Biophys. Acta,1971

4. Harada T. 1965. A new method using test paper containing chromogenic substrates of hydrolases for the isolation and identification of microorganisms p. 629-634. First International Congress of Food Science and Technology vol. 2. Gordon and Research Science Publishers London.

5. Characterization of Pseudomonas isoamylase by its actions on amylopectin and glycogen: comparison with Aerobacter pullulanase;Harada T.;Biochim. Biophys. Acta,1972

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