Affiliation:
1. Department of Chemistry, Cornell University, Ithaca, New York 14853, USA.
Abstract
The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is described. The first gene in the operon is homologous to transcription activators in the lysR family. The second and third genes (thiK and thiC) have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa) catalyzes the phosphorylation of 4-methyl-5-(beta-hydroxyethyl)thiazole. ThiC (27 kDa) catalyzes the substitution of the pyrophosphate of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate. Transcription of the thiC operon is not regulated by thiamine or 2-methyl-4-amino-5-hydroxymethylpyrimidine and is only slightly repressed by 4-methyl-5-(beta-hydroxyethyl)thiazole.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference31 articles.
1. Basic local alignment search tool;Altschul S. F.;J. Mol. Biol.,1990
2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. D. Serdman J. A. Smith and K. Struhl (ed.). 1987. Current protocols in molecular biology. Greene Publishing Associates and Wiley Interscience New York N.Y.
3. Backstrom A. D. and T. P. Begley. 1993. Unpublished data.
4. Biosynthesis of thiamin. I. The function of the thiE gene product;Backstrom A. D.;J. Am. Chem. Soc.,1995
5. The biosynthesis and degradation of thiamin (vitamin B1);Begley T. P.;Nat. Prod. Rep.,1996
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