Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636

Author:

Rasmussen B A1,Gluzman Y1,Tally F P1

Affiliation:

1. Department of Molecular Biology, American Cyanamid Company, Pearl River, New York 10965.

Abstract

Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than 33% identity with the metalloenzyme from Bacillus cereus 569/H.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference17 articles.

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2. Identification of histidine residues that act as zinc ligands in P-lactamaseII by differential tritium exchange;Baldwin G. S.;Biochem. J.,1979

3. BLactamase-mediated imipenem resistance in Bacteroides fragilis;Cuchural G. J.;Antimicrob. Agents Chemother.,1986

4. Gold L. and G. Stormo. 1987. Translation initiation p. 1302-1307. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and E. H. Umbarger (ed.) Escherichia coli and Salmonella typhimurium. American Society for Microbiology Washington D.C.

5. Cloning and sequencing of the metallothioprotein - lactamase II gene of Bacillus cereus 569/H in Escherichia coli;Hussain M.;J. Bacteriol.,1985

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