Complementation of a Treponema denticola flgE Mutant with a Novel Coumermycin A1-Resistant T. denticola Shuttle Vector System-Reference-Cited by-同舟云学术

Complementation of a Treponema denticola flgE Mutant with a Novel Coumermycin A1-Resistant T. denticola Shuttle Vector System

Published:2002-04 Issue:4 Volume:70 Page:2233-2237
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Chi Bo¹,Limberger Ronald J.²,Kuramitsu Howard K.¹

Affiliation:

1. Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214

2. David Axelrod Institute of Public Health, New York State Department of Health, Albany, New York 12201

Abstract

ABSTRACT By using the mutated gyrB gene from a spontaneous coumermycin A1-resistant Treponema denticola , an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to coumermycin was constructed. The complete T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed into the T. denticola ATCC 33520 flgE erythromycin-resistant knockout mutant HL210. The coumermycin-resistant transformants were motile and restored FlgE activity. This complementation system should prove useful in studying the virulence factors of T. denticola and uncultivable pathogenic spirochetes.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.70.4.2233-2237.2002

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