PCR Protocol for Specific Identification of Candida nivariensis , a Recently Described Pathogenic Yeast-Reference-Cited by-同舟云学术

PCR Protocol for Specific Identification of Candida nivariensis , a Recently Described Pathogenic Yeast

Published:2005-12 Issue:12 Volume:43 Page:6194-6196
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Alcoba-Flórez Julia¹²,del Pilar Arévalo María³,González-Paredes F. Javier²,Cano Josep⁴,Guarro Josep⁴,Pérez-Roth Eduardo²,Méndez-Álvarez Sebastián²⁵

Affiliation:

1. Department of Microbiology, Hospital Universitario N. S. de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain

2. Research Institute, Hospital Universitario N. S. de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain

3. Department of Public Health, School of Medicine, University of La Laguna, Tenerife, Spain

4. Unitat de Microbiologia, Facultat de Medicina i Cièncias de la Salut, Universitat Rovira i Virgili, Reus, Tarragona, Spain

5. Department of Cellular Biology & Microbiology, University of La Laguna, Canary Islands, Spain

Abstract

ABSTRACT Candida nivariensis is a recently described pathogenic yeast closely related to Candida glabrata . We developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rRNA gene for the rapid identification of C. nivariensis. PCR with these primers amplified a 206-bp amplicon from C. nivariensis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.12.6194-6196.2005

Reference12 articles.

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4. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene

5. Kurtzman, C. P., and J. W. Fell. 1998. Summary of species characteristics, p.915-947. In C. P. Kurtzman and J. W. Fell (ed.), The yeasts, a taxonomic study, 4th ed. Elsevier Science B. V., Amsterdam, The Netherlands.

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