Affiliation:
1. Department of Microbiology, All India Institute of Medical Sciences
2. International Centre for Genetic Engineering and Biotechnology, New Delhi, India
Abstract
ABSTRACT
Mycoplasma pneumoniae
is the causative agent of primary atypical pneumonia in humans. Adherence of
M. pneumoniae
to host cells requires several adhesin proteins, such as P1, P30, and P116. A major limitation in developing a specific diagnostic test for
M. pneumoniae
is the inability to express adhesin proteins in heterologous expression systems due to unusual usage of the UGA stop codon, leading to premature termination of these proteins in
Escherichia coli
. In the present study, we successfully expressed the C-terminal (P1-C1) and N-terminal (P1-N1) regions of the P1 protein in
E. coli
. On screening these recombinant proteins with sera from
M. pneumoniae
-infected patients, only the P1-C1 protein was found to be immunogenic. This protein can be used as an antigen for immunodiagnosis of
M. pneumoniae
infection, as well as in adherence inhibition studies to understand the pathophysiology of the disease.
Publisher
American Society for Microbiology
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