Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections-Reference-Cited by-同舟云学术

Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections

Published:2005-10 Issue:10 Volume:43 Page:5362-5365
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Vidal Maricel¹,Kruger Eileen¹,Durán Claudia¹,Lagos Rosanna²,Levine Myron³,Prado Valeria¹,Toro Cecilia¹,Vidal Roberto¹

Affiliation:

1. Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile

2. Centro para Vacunas en Desarrollo, Santiago, Chile

3. Center for Vaccine Development, University of Maryland, Baltimore, Maryland

Abstract

ABSTRACT We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli . This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing ( stx 1 , stx 2 , and eae ), enteropathogenic ( eae and bfp ), enterotoxigenic ( stII and lt ), enteroinvasive ( virF and ipaH ), enteroaggregative ( aafII ), and diffuse adherent ( daaE ) Escherichia coli in stool samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.10.5362-5365.2005

Reference20 articles.

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