Purification of Pneumolysin-Reference-Cited by-同舟云学术

Purification of Pneumolysin

Published:1971-10 Issue:4 Volume:4 Page:388-392
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Shumway Clare N.¹,Klebanoff Seymour J.²

Affiliation:

1. Department of Pediatrics, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219

2. Department of Medicine, U. S. Public Health Service Hospital, University of Washington School of Medicine, Seattle, Washington 98114

Abstract

A procedure for the purification of pneumolysin has been developed. Its use results in a 96-fold increase in specific activity from the original crude pneumococcal extract to the final purified product. The purified material is heat-labile and is inactivated by trypsin and cholesterol; inactivation by p -hydroxymercuribenzoate can be reversed by mercaptoethanol. Intravenous administration of the material to rabbits results in crenation, increased osmotic fragility, and intravascular lysis of the erythrocytes of the animal.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.4.4.388-392.1971

Reference15 articles.

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2. Pneumococcal hemolysin;Cohen B.;J. Bacteriol.,1942

3. Pneumococcus hemotoxin;Cole R.;J. Exp. Med.,1914

4. Pnoumococcal hemolysin: its cxtracellular nature, production and properties;Cowan S. T.;J. Pathol. Bacteriol.,1934

5. Toxic and immunological properties of pneumococcal hemolysin;Halbert S. P.;Bull. Johns Hopkins Hospital,1946

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