Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to d -Mannose Receptors-Reference-Cited by-同舟云学术

Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to d -Mannose Receptors

Published:2005-02 Issue:2 Volume:73 Page:1226-1231
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Schwan William R.¹,Beck Michael T.¹,Hultgren Scott J.²,Pinkner Jerry²,Woolever Nathan L.¹,Larson Thomas¹

Affiliation:

1. University of Wisconsin-La Crosse, La Crosse, Wisconsin

2. Washington University, St. Louis, Missouri

Abstract

ABSTRACT A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to d -mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli .

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.73.2.1226-1231.2005

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