Acid adaptation of Escherichia coli O157:H7 increases survival in acidic foods

Author:

Leyer G J1,Wang L L1,Johnson E A1

Affiliation:

1. Departments of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706, USA.

Abstract

Escherichia coli O157:H7 was adapted to acid by culturing for one to two doublings at pH 5.0. Acid-adapted cells had an increased resistance to lactic acid, survived better than nonadapted cells during a sausage fermentation, and showed enhanced survival in shredded dry salami (pH 5.0) and apple cider (pH 3.4). Acid adaptation is important for the survival of E. coli O157:H7 in acidic foods and should be considered a prerequisite for inocula used in food challenge studies.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference22 articles.

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2. An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider;Besser R. E.;JAMA,1993

3. Update: multistate outbreak of Escherichia coli O157:H7 infections from hamburgers-western United States, 1992-1993;Centers for Disease Control and Prevention.;Morbid. Mortal. Weekly Rep.,1993

4. Escherichia coli O157:H7 outbreak linked to commercially distributed dry-cured salami-Washington and California;Centers for Disease Control and Prevention.;Morbid. Mortal. Weekly Rep.,1995

5. Salmonella acid shock proteins are required for the adaptive acid tolerance response;Foster J. W.;J. Bacteriol.,1991

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