Affiliation:
1. Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich,1 and
2. Krankenhaus Dritter Orden, 1 Medical Ward, 80638 Munich,2 Germany
Abstract
ABSTRACT
We isolated a hitherto undescribed microorganism from a patient with endocarditis. The microscopic appearance, a negative catalase reaction, and growth as satellite colonies next to
Staphylococcus epidermidis
suggested that this microorganism is a member of the genus
Abiotrophia
, formerly known as nutritionally variant streptococci. However, the clinical isolate described herein differed markedly from the known
Abiotrophia
spp.,
A. adiacens
and
A. defectiva
, in terms of its (i) biochemical properties, (ii) restricted growth temperature range, (iii) whole-cell lysate polypeptide profile, and (iv) unique nutritional requirements. In contrast to the type strains of
A. adiacens
and
A. defectiva
, which used
l
-cysteine and pyridoxal hydrochloride as growth factors, the growth of the clinical isolate was only supported by
l
-cysteine hydrochloride and not by pyridoxal hydrochloride when the organism was tested in Todd-Hewitt or casein-soy peptone broth. Comparative 16S rRNA gene sequence analysis revealed that the microorganism was a member of the genus
Abiotrophia
and was most closely related to
A. adiacens
(96.9% homology). Phenotypic and phylogenetic data are consistent with the assumption of a new species within the genus
Abiotrophia
, for which we propose the name
Abiotrophia elegans
sp. nov. The unique nutritional requirements of this strain are of importance for diagnostic laboratories. The media of blood culture systems supplemented only with pyridoxal hydrochloride as a growth factor may fail to promote the growth of
A. elegans
sp. nov., and thus, these systems might not detect this microorganism as a possible cause of endocarditis.
Publisher
American Society for Microbiology
Cited by
76 articles.
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