Evaluation of an Upgraded Version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 Test for HIV-1 Load Quantification

Author:

Damond F.1,Avettand-Fenoel V.23,Collin G.1,Roquebert B.14,Plantier J. C.5,Ganon A.2,Sizmann D.6,Babiel R.6,Glaubitz J.6,Chaix M. L.23,Brun-Vezinet F.14,Descamps D.14,Rouzioux C.23

Affiliation:

1. APHP, Laboratoire de Virologie, Hopital Bichat Claude Bernard, Paris, France

2. APHP, Laboratoire de Virologie, Hopital Necker, Paris, France

3. EA3620, Université Rene Descartes, Paris 5, France

4. EA4409, Université Denis Diderot, Paris 7, France

5. Laboratoire de Virologie, Institut de Biologie Clinique, Hôpital Ch. Nicolle, Rouen, France

6. Roche Molecular Diagnostics Ltd., Rotkreuz, Switzerland

Abstract

ABSTRACT We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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