Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice-Reference-Cited by-同舟云学术

Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice

Published:2009-05 Issue:9 Volume:75 Page:2964-2968
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Simon Carola¹,Herath Judith¹,Rockstroh Stephanie¹,Daniel Rolf¹

Affiliation:

1. Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstrasse 8, 37077 Göttingen, Germany

Abstract

ABSTRACT Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02644-08

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