Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle-Reference-Cited by-同舟云学术

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

Published:2003-04 Issue:4 Volume:41 Page:1726-1729
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

DeGraves Fred J.¹,Gao Dongya¹,Hehnen Hans-Robert²,Schlapp Tobias²,Kaltenboeck Bernhard¹

Affiliation:

1. Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519

2. Bayer AG, Animal Health, Alfred-Nobel-Strasse 50, D-40789 Monheim, Germany

Abstract

ABSTRACT Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti- Chlamydia antibodies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.4.1726-1729.2003

Reference12 articles.

1. Molecular Diagnosis of Chlamydia pneumoniae Infection

2. DeGraves F. J. D. Gao and B. Kaltenboeck. 2003. A high-sensitivity quantitative PCR platform. BioTechniques 34: 106-115.

3. Domeika, M., A. Ganusauskas, M. Bassiri, G. Fröman, and P.-A. Mårdh. 1994. Comparison of polymerase chain reaction, direct immunofluorescence, cell culture and enzyme immunoassay for the detection of Chlamydia psittaci in bull semen. Vet. Microbiol.42:273-280.

4. Galen R. S. 1986. Use of predictive value theory in clinical immunology p 966-970. In N. R. Rose H. Friedman and J. L. Fahey (ed.) Manual of clinical laboratory immunology 3rd ed. American Society for Microbiology Washington D.C.

5. Huang, J., F. J. DeGraves, D. Gao, P. Feng, T. Schlapp, and B. Kaltenboeck. 2001. Quantitative detection of Chlamydia spp. by fluorescent PCRs in the LightCycler. BioTechniques30:151-157.

Cited by 74 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Identification of Chlamydia abortus and Chlamydia pecorum in water buffaloes (Bubalus bubalis) and cows cohabitating the same herd;Buffalo Bulletin;2023-12-31

2. Effects of dam milk yield and milk composition on birth weight and growth performance of Anatolian buffalo calves;Buffalo Bulletin;2023-12-31

3. Genome Dynamics and Temperature Adaptation During Experimental Evolution of Obligate Intracellular Bacteria;Genome Biology and Evolution;2023-07-29

4. chlamydial infections of livestock and poultry;CABI Compendium;2022-01-07

5. Fetal Disease and Abortion;Bovine Reproduction;2021-05-03