Simple method for quantitation of viable mycoplasmas

Author:

Albers A C,Fletcher R D

Abstract

A rapid, simple, and inexpensive method for quantitation of viable mycoplasmas is described. Serial dilutions were made in sterile microtiter plates with standard microtiter equipment. The results were multiplied by a factor of 2.38 to obtain colony-forming units comparable to those obtained with the laborious glass pipette-tube dilution method.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference7 articles.

1. Adherence of Mycoplasma pneumoniae to glass surfaces;Feldner J.;Infect. Immun.,1979

2. Inactivation of mycoplasmas by long-chain alcohols;Fletcher R. D.;Antimicrob. Chemother.,1981

3. Inhibition of Mycoplasma pneumoniae by actinomycin D;Fletcher R. D.;Antimicrob. Chemother.,1973

4. Freundt E. A. 1974. The mycoplasmas p. 929-955. In R. E. Buchanan and N. E. Gibbons (ed.) Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.

5. Adherence of Mycoplasma gallisepticum to glass;Kahane I.;J. Gen. Microbiol.,1979

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