One-step purification of nisin A by immunoaffinity chromatography-Reference-Cited by-同舟云学术

One-step purification of nisin A by immunoaffinity chromatography

Published:1997-12 Issue:12 Volume:63 Page:4990-4992
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Suárez A M¹,Azcona J I¹,Rodríguez J M¹,Sanz B¹,Hernández P E¹

Affiliation:

1. Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anagea@eucmax.sim.ucm.es

Abstract

The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N-hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.63.12.4990-4992.1997

Reference21 articles.

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