GenoType NTM-DR Performance Evaluation for Identification of Mycobacterium avium Complex and Mycobacterium abscessus and Determination of Clarithromycin and Amikacin Resistance

Author:

Huh Hee Jae1,Kim Su-Young2,Shim Hyang Jin3,Kim Dae Hun2,Yoo In Young1,Kang On-Kyun1,Ki Chang-Seok4,Shin So Youn5,Jhun Byung Woo2,Shin Sung Jae678,Daley Charles L.910,Koh Won-Jung2,Lee Nam Yong1

Affiliation:

1. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

2. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

3. Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, South Korea

4. Green Cross Genome, Yongin, South Korea

5. Korean Institute of Tuberculosis, Cheongju, South Korea

6. Department of Microbiology, Yonsei University College of Medicine, Seoul, South Korea

7. Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, Seoul, South Korea

8. Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea

9. Division of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, Colorado, USA

10. Department of Medicine, University of Colorado, Aurora, Colorado, USA

Abstract

We evaluated the GenoType NTM-DR (NTM-DR) line probe assay for identifying Mycobacterium avium complex (MAC) species and Mycobacterium abscessus subspecies and for determining clarithromycin and amikacin resistance. Thirty-eight reference strains and 145 clinical isolates (58 MAC and 87 M. abscessus isolates), including 54 clarithromycin- and/or amikacin-resistant strains, were involved.

Funder

National Research Foundation of Korea

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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