Affiliation:
1. Department of Infectious Diseases, Leiden University Medical Center, Leiden,1 and
2. Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven,2 The Netherlands
Abstract
ABSTRACT
During colonization of the respiratory tract by
Bordetella pertussis
, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of
B. pertussis
to cells of the human bronchial epithelial cell line NCI-H
292
and of the laryngeal epithelial cell line HEp-2. Using
B. pertussis
mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of
B. pertussis
to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of
B. pertussis
to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
79 articles.
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