Cytosolic Gag p24 as an Index of Productive Entry of Human Immunodeficiency Virus Type 1-Reference-Cited by-同舟云学术

Cytosolic Gag p24 as an Index of Productive Entry of Human Immunodeficiency Virus Type 1

Published:1998-03 Issue:3 Volume:72 Page:2208-2212
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Maréchal Valérie¹,Clavel François²,Heard Jean Michel¹,Schwartz Olivier¹

Affiliation:

1. Laboratoire Rétrovirus et Transfert Génétique1 and

2. Unitéd’oncologie Virale,2 CNRS URA 1157, Institut Pasteur, 75724 Paris, France

Abstract

ABSTRACT We have investigated the cellular uptake of Gag p24 shortly after exposure of cells to human immunodeficiency virus (HIV) particles. In the absence of envelope glycoprotein on virions or of viral receptors or coreceptors at the cell surface, p24 was incorporated in intracellular vesicles but not detected in the cytosolic subcellular fraction. When appropriate envelope-receptor interactions could occur, the nonspecific vesicular uptake was still intense and cytosolic p24 represented 10 to 40% of total intracellular p24. The measurement of cytosolic p24 early after exposure to HIV type 1 is a reliable assay for investigating virus entry and early events leading to authentic cell infection.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.72.3.2208-2212.1998

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