Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci-Reference-Cited by-同舟云学术

Novel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci

Published:2006-03 Issue:3 Volume:44 Page:1141-1144
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

McClure Jo-Ann¹,Conly John M.¹²³⁴⁵,Lau Vicky¹,Elsayed Sameer²³⁵,Louie Thomas³⁴,Hutchins Wendy³,Zhang Kunyan¹²³⁴⁵

Affiliation:

1. Centre for Antimicrobial Resistance, Calgary Health Region/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada

2. Departments of Pathology & Laboratory Medicine

3. Microbiology and Infectious Diseases

4. Medicine, University of Calgary, Calgary, Alberta, Canada

5. Calgary Laboratory Services, Calgary, Alberta, Canada

Abstract

ABSTRACT We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.3.1141-1144.2006

Reference31 articles.

1. Begier, E. M., K. Frenette, N. L. Barrett, P. Mshar, S. Petit, D. J. Boxrud, K. Watkins-Colwell, S. Wheeler, E. A. Cebelinski, A. Glennen, D. Nguyen, and J. L. Hadler. 2004. A high-morbidity outbreak of methicillin-resistant Staphylococcus aureus among players on a college football team, facilitated by cosmetic body shaving and turf burns. Clin. Infect. Dis.39:1446-1453.

2. Beilman, G. J., G. Sandifer, D. Skarda, B. Jensen, S. McAllister, G. Killgore, and A. Srinivasan. 2005. Emerging infections with community-associated methicillin-resistant Staphylococcus aureus in outpatients at an army community hospital. Surg. Infect. (Larchmont)6:87-92.

3. Conly, J., M. Gilbert, K. Zhang, D. Gregson, S. Elsayed, M. Mulvey, K. Laupland, T. Louie, H. Rabin, B. Baylis, and P. Boiteau. 2005. Rapidly progressive fatal necrotizing pneumonitis (FNP) 2° to Panton-Valentine leukocidin (PVL) + SCCmec type IVa community-acquired methicillin-resistant S. aureus (CAMRSA)—a harbinger of the future? Can. J. Infect. Dis. Med. Microbiol.16:109.

4. Deurenberg, R. H., C. Vink, C. Driessen, M. Bes, N. London, J. Etienne, and E. E. Stobberingh. 2004. Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR. FEMS Microbiol. Lett.240:225-228.

5. Etienne, J. 2005. Panton-Valentine leukocidin: a marker of severity for Staphylococcus aureus infection? Clin. Infect. Dis.41:591-593.

Cited by 289 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Prevalence of Multidrug-Resistant Staphylococcus aureus in Meals Served at Hospitals;Egyptian Journal of Veterinary Sciences;2025-07-01

2. Unravelling the complex mechanisms of multidrug resistance in bovine mastitis pathogens: Insights into antimicrobial resistance genes, biofilm dynamics, and efflux systems;Microbial Pathogenesis;2024-10

3. Green synthesized silver nanoparticles from Ocimum sanctum: A potent inhibitor of biofilm forming ability and efflux pumps in bacteria causing bovine mastitis;Microbial Pathogenesis;2024-10

4. Vaginal colonization with virulent and methicillin resistant Staphylococcus aureus among Ugandan women in Labour;BMC Microbiology;2024-08-19

5. Panton-Valentine leucocidin gene in methicillin resistant Staphylococcus aureus isolated from tertiary care hospital in Nepal;The Journal of Infection in Developing Countries;2024-07-29