Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid-Reference-Cited by-同舟云学术

Intravitam Diagnosis of Human Rabies by PCR Using Saliva and Cerebrospinal Fluid

Published:1998-04 Issue:4 Volume:36 Page:1117-1121
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Crepin P.¹,Audry L.¹,Rotivel Y.¹,Gacoin A.²,Caroff C.²,Bourhy H.¹

Affiliation:

1. Rabies Unit, Institut Pasteur, Paris,1 and

2. Regional and University Hospital, Rennes,2 France

Abstract

ABSTRACT An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.4.1117-1121.1998

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