An Immunological Assay for Detection and Enumeration of Thermophilic Biomining Microorganisms-Reference-Cited by-同舟云学术

An Immunological Assay for Detection and Enumeration of Thermophilic Biomining Microorganisms

Published:1994-09 Issue:9 Volume:60 Page:3470-3473
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Amaro Ana M.¹,Hallberg Kevin B.²,Lindström E. Börje²,Jerez Carlos A.¹

Affiliation:

1. Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago, Chile

2. Department of Applied Cell and Molecular Biology, University of Umeå, S-901 87 Umeå, Sweden

Abstract

A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile Thiobacillus caldus and the thermophilic archaeon Sulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.9.3470-3473.1994

Reference19 articles.

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2. Brierley J. A. and C. L. Brierley. 1986. Microbial mining using thermophilic microorganisms p. 279-305. In T. D. Brock (ed.) Thermophiles: general molecular and applied microbiology. John Wiley & Sons Inc. New York.

3. Tolerance to temperature and water quality for bacterial oxidation: the benefits of BacTech's moderately thermophilic culture;Budden J. R;FEMS Microbiol. Rev.,1993

4. Hallberg K. B. and E. B. Lindstrom. Characterization of Thiobacillus caldus sp. nov. a moderately thermophilic acidophile. Microbiology (United Kingdom) in press.

5. The acidophilic Thiobacilli and other acidophilic bacteria that share their habitat. Annu;Harrison A. P.;Rev. Microbiol.,1984

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