Development of a Flow Chart for Identification of Gram-Positive Anaerobic Cocci in the Clinical Laboratory

Author:

Song Yuli1,Liu Chengxu1,Finegold Sydney M.234

Affiliation:

1. Research Service

2. Infectious Diseases Section, Veterans Administration Medical Center West Los Angeles

3. Department of Medicine

4. Department of Microbiology, Immunology, and Molecular Genetics, University of California—Los Angeles School of Medicine, Los Angeles, California

Abstract

ABSTRACT Gram-positive anaerobic cocci (GPAC) are a heterogeneous group of organisms that are isolated from clinical specimens more often than any group of anaerobic bacteria except Bacteroides species, yet many strains are still difficult or impossible to identify in the diagnostic laboratory. In this study, a total of 124 strains, including 13 reference strains of GPAC species and 111 isolates that had been recovered from clinical specimens previously and identified by 16S rRNA gene sequencing, were subjected to biochemical characterization. Based on the results, a short biochemical scheme that involves the minimum essential biochemical tests for accurate identification of clinically important GPAC was developed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

1. Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals

2. Finegold S. M. 1977. Anaerobic bacteria in human disease. Academic Press New York NY.

3. Numbers and types of anaerobic bacteria isolated from clinical specimens since 1960

4. Jousimies-Somer H. R. P. Summanen D. M. Citron E. J. Baron H. M. Wexler and S. M. Finegold. 2002. Wadsworth anaerobic bacteriology manual 6th ed. Star Publishing Co. Belmont CA.

5. Gram-Positive Anaerobic Cocci

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