Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and Borrelia Species-Specific Tests Based on Whole-Cell and Recombinant Antigens-Reference-Cited by-同舟云学术

Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and Borrelia Species-Specific Tests Based on Whole-Cell and Recombinant Antigens

Published:2018-11 Issue:11 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kodym Petr¹,Kurzová Zuzana¹,Berenová Dagmar¹,Pícha Dušan²,Smíšková Dita²,Moravcová Lenka²,Malý Marek³

Affiliation:

1. Department of Zoonoses with Natural Focality, Centre for Epidemiology and Microbiology, National Institute of Public Health, Prague, Czech Republic

2. Department of Infectious Diseases, 2nd Faculty of Medicine, Charles University and Na Bulovce Hospital, Prague, Czech Republic

3. Department of Biostatistics, National Institute of Public Health, Prague, Czech Republic

Abstract

The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme immunoassays (EIAs) (TestLine), Liaison chemiluminescence (DiaSorin), ELISA-Viditest (Vidia), EIA, and Blot-Line (TestLine) using recombinant antigens.

Funder

Grant Agency of the Ministry of Health of the Czech republic

Ministry of Health of the Czech Republic

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00601-18

Reference25 articles.

1. Epidemiology of Lyme Borreliosis

2. Clinical Manifestations and Diagnosis of Lyme Borreliosis