Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli-Reference-Cited by-同舟云学术

Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli

Published:2009-06 Issue:6 Volume:47 Page:1915-1917
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Barletta F.¹,Ochoa T. J.¹²,Ecker L.³,Gil A. I.³,Lanata C. F.³,Cleary T. G.²

Affiliation:

1. Instituto de Medicina Tropical, Universidad Peruana Cayetano Heredia, Lima, Peru

2. Center for Infectious Diseases, University of Texas School of Public Health, Houston, Texas

3. Instituto de Investigación Nutricional, Lima, Peru

Abstract

ABSTRACT Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00608-09

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