Affiliation:
1. McCollum-Pratt Institute, Johns Hopkins University, Baltimore, Maryland 21218.
Abstract
Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been partially characterized in vitro and in vivo, and it appears to be identical to the deoxynucleotide kinase isolated from T4-infected Escherichia coli. These results prove the earlier contention that the phosphorylation of three dissimilar deoxynucleotides (5-hydroxymethyldeoxycytidylate, dTMP, and dGMP), to the exclusion of most others, is catalyzed by a single protein.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference24 articles.
1. Adams M. H. 1959. Bacteriophages p. 473-485. Interscience Publishers Inc. New York.
2. The enzymology of virus-infected bacteria. IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2;Bello L. J.;J. Biol. Chem.,1963
3. Deoxyribonucleotide kinase in normal and virus-infected Escherichia coli;Bessman M. J.;J. Biol. Chem.,1959
4. A complementation analysis of the restriction and modification of DNA in Escherichia coli;Boyer H. W.;J. Mol. Biol.,1969
5. Sequence organization and control of transcription in the bacteriophage T4 tRNA region;Broida J.;J. Mol. Biol.,1985
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献