Specific Xylan Activity Revealed for AA9 Lytic Polysaccharide Monooxygenases of the Thermophilic Fungus Malbranchea cinnamomea by Functional Characterization-Reference-Cited by-同舟云学术

Specific Xylan Activity Revealed for AA9 Lytic Polysaccharide Monooxygenases of the Thermophilic Fungus Malbranchea cinnamomea by Functional Characterization

Published:2019-12 Issue:23 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hüttner Silvia¹²^ORCID,Várnai Anikó³^ORCID,Petrović Dejan M.³,Bach Cao Xuan⁴,Kim Anh Dang Thi⁴,Thanh Vu Nguyen⁴,Eijsink Vincent G. H.³^ORCID,Larsbrink Johan¹²^ORCID,Olsson Lisbeth¹²

Affiliation:

1. Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden

2. Wallenberg Wood Science Center, Chalmers University of Technology, Gothenburg, Sweden

3. Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway

4. Centre for Industrial Microbiology, Food Industries Research Institute, Hanoi, Vietnam

Abstract

The Malbranchea cinnamomea LPMOs ( Mc AA9s) showed activity on a broad range of soluble and insoluble substrates, suggesting their involvement in various steps of biomass degradation besides cellulose decomposition. Our results indicate that the fungal AA9 family is more diverse than originally thought and able to degrade almost any kind of plant cell wall polysaccharide. The discovery of an AA9 that preferentially cleaves xylan enhances our understanding of the physiological roles of LPMOs and enables the use of xylan-specific LPMOs in future applications.

Funder

Research Council of Norway

Vetenskapsrådet

Wallenberg Wood Science Center

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01408-19

Reference50 articles.

1. Recent insights into lytic polysaccharide monooxygenases (LPMOs)