Cellular Fatty Acid Composition of Lautropia mirabilis

Author:

Daneshvar Maryam I.1,Douglas Michael P.1,Weyant Robbin S.1

Affiliation:

1. Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333

Abstract

ABSTRACT Ten strains of Lautropia mirabilis (ATCC 51599 T and nine phenotypically similar clinical isolates) were examined for cellular fatty acid (CFA) composition to evaluate their chemical relatedness to known bacterial species and groups. The CFAs were liberated from whole cells by base hydrolysis, methylated, and analyzed by gas-liquid chromatography. CFA profiles were generated by using a commerical software package (MIDI, Newark, Del.). All strains tested had an identical CFA profile characterized by major amounts of 16:1ω7c (41%) and 16:0 (44%); smaller amounts (1 to 4%) of 3-OH-10:0, 12:0, 14:0, 15:0, and 18:1 ω7c; trace amounts (<1%) of 10:0, 18:2 and 18:0; and no cyclopropane acids. This profile was similar to the CFA profiles of Acidovorax delafieldii, Comamonas terrigena , and strains of an unclassified Centers for Disease Control group designated weak oxidizer group 1. CFA analysis, when supplemented by phenotypic characterization, is useful for the identification of L. mirabilis isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference5 articles.

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