An Antiphagocytic Factor Associated with Group E Streptococcus

Abstract

Group E streptococci (GES) grown in Tryptose phosphate broth (TPB) were highly susceptible to nonspecific phagocytosis by porcine leukocytes, whereas GES grown in serum-supplemented TPB (STPB) were resistant to nonspecific phagocytosis. It was concluded that serum supplementation induced the elaboration of an antiphagocytic factor (APF). The APF was distinct from the group-specific and type-specific polysaccharide antigens, and it was destroyed by trypsin. Electron photomicrographs revealed that GES grown in STPB possessed a fimbriated surface component similar to M protein-positive stains of group A streptococcus. The surface component was absent when GES were propagated in unfortified TPB. The APF was neutralized specifically by sera from swine immune to streptococcic lymphadenitis (SLS), a disease caused by GES. It was hypothesized that the APF was associated with the pathogenicity of GES and that it was involved in the induction of protective immunity to SLS.