Global Regulation of the Response to Sulfur Availability in the Cheese-Related Bacterium Brevibacterium aurantiacum

Author:

Forquin Marie-Pierre12,Hébert Agnès3,Roux Aurélie4,Aubert Julie5,Proux Caroline6,Heilier Jean-François47,Landaud Sophie1,Junot Christophe4,Bonnarme Pascal1,Martin-Verstraete Isabelle28

Affiliation:

1. INRA-AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires, Centre de Biotechnologies Agro-Industrielles, 78850 Thiverval-Grignon, France

2. Institut Pasteur, Laboratoire Pathogenèse des Bactéries Anaérobies, 25-28 Rue du Docteur Roux, 75724 Paris Cedex 15, France

3. INRA-AgroParisTech, UMR 1319 Micalis, Centre de Biotechnologies Agro-Industrielles, 78850 Thiverval-Grignon, France

4. CEA, Service de Pharmacologie et d'Immunoanalyse, DSV/iBiTec-S, CEA/Saclay, 91191 Gif-sur-Yvette Cedex, France

5. INRA-AgroParisTech, UMR 518 Mathématiques et Informatiques Appliquées, Paris, France

6. Institut Pasteur, Plate-forme Puces à ADN, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France

7. Université Catholique de Louvain, Louvain Centre for Toxicology and Applied Pharmacology, Brussels, Belgium

8. Université Paris 7-Denis Diderot, 75205 Paris, France

Abstract

ABSTRACT In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum . In agreement with the growth of B. aurantiacum in the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway, thiolation pathways that produce cysteine ( cysE and cysK ) or homocysteine ( metX and metY ) from sulfide, at least one gene of the transsulfuration pathway ( aecD ), and genes encoding three MetE-type methionine synthases. We also compared the expression profiles of B. aurantiacum ATCC 9175 during sulfur starvation or in the presence of sulfate. Under sulfur starvation, 690 genes, including 21 genes involved in sulfur metabolism and 29 genes encoding amino acids and peptide transporters, were differentially expressed. We also investigated changes in pools of sulfur-containing metabolites and in expression profiles after growth in the presence of sulfate, cystine, or methionine plus cystine. The expression of genes involved in sulfate assimilation and cysteine synthesis was repressed in the presence of cystine, whereas the expression of metX , metY , metE1 , metE2 , and BL613 , encoding a probable cystathionine-γ-synthase, decreased in the presence of methionine. We identified three ABC transporters: two operons encoding transporters were transcribed more strongly during cysteine limitation, and one was transcribed more strongly during methionine depletion. Finally, the expression of genes encoding a methionine γ-lyase ( BL929 ) and a methionine transporter ( metPS ) was induced in the presence of methionine in conjunction with a significant increase in volatile sulfur compound production.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference58 articles.

1. Identification and Functional Analysis of the Gene Encoding Methionine-γ-Lyase in Brevibacterium linens

2. Arfi, K., F. Amarita, H. E. Spinnler, and P. Bonnarme. 2003. Catabolism of volatile sulfur compounds precursors by Brevibacterium linens and Geotrichum candidum, two microorganisms of the cheese ecosystem. J. Biotechnol. 105:245-253.

3. Auger, S., M. P. Gomez, A. Danchin, and I. Martin-Verstraete. 2005. The PatB protein of Bacillus subtilis is a C-S-lyase. Biochimie 87:231-238.

4. Benjamini, Y., and Y. Hochberg. 1995. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc. B Stat. Methods 57:289-300.

5. Bockelmann, W. 2003. Smear ripened cheeses, p. 391-401. In H. Roginski, J. W. Fuquay, and P. F. Fox (ed.), Encyclopedia of dairy sciences. Academic Press, Inc., New York, NY.

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