Improved Method for Bacterial Cell Capture after Flow Cytometry Cell Sorting-Reference-Cited by-同舟云学术

Improved Method for Bacterial Cell Capture after Flow Cytometry Cell Sorting

Published:2010-11 Issue:21 Volume:76 Page:7352-7355
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Guillebault D.¹²,Laghdass M.¹²,Catala P.¹²,Obernosterer I.¹²,Lebaron P.¹²

Affiliation:

1. UPMC Université Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France

2. CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France

Abstract

ABSTRACT Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly- l -lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00621-10

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