The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions-Reference-Cited by-同舟云学术

The Evolution of Azole Resistance in Candida albicans Sterol 14α-Demethylase (CYP51) through Incremental Amino Acid Substitutions

Published:2019-05 Issue:5 Volume:63 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Warrilow Andrew G.¹,Nishimoto Andrew T.²³,Parker Josie E.¹,Price Claire L.¹,Flowers Stephanie A.²³,Kelly Diane E.¹,Rogers P. David²³,Kelly Steven L.¹

Affiliation:

1. Institute of Life Science, Swansea University Medical School, Swansea University, Swansea, Wales, United Kingdom

2. Department of Clinical Pharmacy and Translational Science, College of Pharmacy, University of Tennessee Health Science Center, Memphis, Tennessee, USA

3. University of Tennessee Center for Pediatric Experimental Therapeutics, Memphis, Tennessee, USA

Abstract

Recombinant Candida albicans CYP51 (CaCYP51) proteins containing 23 single and 5 double amino acid substitutions found in clinical strains and the wild-type enzyme were expressed in Escherichia coli and purified by Ni 2+ -nitrilotriacetic acid agarose chromatography. Catalytic tolerance to azole antifungals was assessed by determination of the concentration causing 50% enzyme inhibition (IC 50 ) using CYP51 reconstitution assays.

Funder

EPSRC

HHS | National Institutes of Health

EC | European Regional Development Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02586-18

Reference56 articles.

1. Antifungal Resistance and New Strategies to Control Fungal Infections