Affiliation:
1. Division of Infectious Diseases, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298-0049
2. Facultad Huesca, Zaragoza, Spain
Abstract
ABSTRACT
NCCLS document M38-P describes standard parameters for testing the fungistatic activities (MICs) of established agents against filamentous fungi (molds). This study evaluated the in vitro susceptibilities of 15
Aspergillus flavus
isolates, 62
A. fumigatus
isolates, and 10 isolates each of
A. niger
,
A. nidulans
, and
A. terreus
to voriconazole, posaconazole, itraconazole, and amphotericin B by the E-test and NCCLS M38-P microdilution methods. The agreement (within 3 dilutions) between methods for voriconazole was independent of the E-test incubation time (93.3 to 100% for four of five species at both incubation times). In contrast, with amphotericin B, itraconazole, and posaconazole, E-test results were more dependent on the incubation time for certain species. For
A. fumigatus,
posaconazole E-test MICs had better concordance with reference values after 48 h (95.2%) than after 24 h (90%), while the highest agreement for itraconazole MICs was after 24 h (90.3 versus 74.2%) of incubation. Better agreement between the methods was also obtained with 24-h E-test amphotericin B MICs for
A. flavus
(73.3 versus 26.7%) and
A. fumigatus
(96.7 versus 64.5%). E-test MICs of the four agents had the lowest percentages of agreement with reference values for
A. nidulans
(60 to 80%). For isolates for which high MICs were obtained for the four agents by the reference method, high MICs were also obtained by E-test at both 24 and 48 h. The utility of in vitro results of either the E-test or the NCCLS broth microdilution (M38-P) method for
Aspergillus
spp. needs to be established in clinical trials.
Publisher
American Society for Microbiology
Cited by
79 articles.
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