Molecular Analysis of Virulence Profiles and Shiga Toxin Genes in Food-Borne Shiga Toxin-Producing Escherichia coli

Abstract

ABSTRACT In this study, 75 Shiga toxin (Stx)-producing Escherichia coli (STEC) strains originating from foods ( n = 73) and drinking water ( n = 2) were analyzed for their stx genotype, as well as for further chromosome-, phage-, and plasmid-encoded virulence factors. A broad spectrum of stx genes was detected. Fifty-three strains (70.7%) contained stx 2 or stx 2 variants, including stx 2d , mucus-activatable stx 2d , stx 2e , and stx 2g . Seven strains (9.3%) harbored stx 1 or stx 1c , and 15 strains (20.0%) carried both stx 2 and/or stx 2 variants and stx 1 or stx 1c . Beside stx , the most abundant accessory virulence markers in STEC food isolates were iha (57.3%), ehxA (40.0%), espP (28.0%), and subAB (25.3%). Only four strains were eae positive; three of these belonged to the serogroups O26, O103, and O157 and contained a typical enterohemorrhagic E. coli virulence spectrum. The results of this study show that a number of STEC strains that occur in foods appear to be pathogenic for humans, based on their virulence profiles. Analysis of stx subtypes and detection of additional virulence factors in eae -negative strains may help to better assess the risk of such strains for causing human infection.