Affiliation:
1. Venereal Disease Research Laboratory, Venereal Disease Branch, Communicable Disease Center, U.S. Public Health Service, Atlanta, Georgia
Abstract
Direct smears from female patients have been considered unreliable for the detection of
Neisseria gonorrhoeae
by fluorescent-antibody (FA) methods because of inadequate background contrast of the fluorescent-stained smears and a scarcity of organisms on the smear. Evans blue dye employed as a counterstain eliminated the nonspecific background staining and increased the reliability of the direct FA procedure. Direct smears demonstrating positive fluorescence were obtained from 86% of a group of culturally positive named female contacts. The FA-counterstain technique is as sensitive as the presently recommended cultural procedures.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference9 articles.
1. CHERRY W. B. M. GOLDMAN AND T. R. CARSKI. 1960. Fluorescent antibody techniques in the diagnosis of communicable diseases. Public Health Service Publ. No. 729.
2. Identification of Neisseria gonorrhoeae by means of fluorescent antibodies;DEACON W. E.;Proc. Soc. Exp. Biol. Med.,1959
3. Fluorescent antibody tests for detection of the gonococcus in women;DEACON W. E.;Public Health Rep. (U.S.),1960
4. Chelated azo dyes used as counterstains in the fluorescent antibody technic;HALL C. T.;Zentr. Bakteriol. Parasitenk. Abt. I. Orig.,1962
5. Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation;KELLOGG D. S.;J. Bacteriol.,1963