Comparison of Broth Microdilution Method Using Haemophilus Test Medium and Agar Dilution Method for Susceptibility Testing of Eikenella corrodens

Author:

Alcalá Luis1,García-Garrote Fernando1,Cercenado Emilia1,Peláez Teresa1,Ramos Gema1,Bouza Emilio1

Affiliation:

1. Servicio de Microbiologı́a y Enfermedades Infecciosas, Hospital General Universitario “Gregorio Marañón,” Madrid, Spain

Abstract

ABSTRACT Susceptibility testing of Eikenella corrodens is usually performed by a Mueller-Hinton sheep blood agar dilution (AD) method. However, this method is impractical for testing only a few strains. We compared AD with the broth microdilution method using Haemophilus test medium (HTM) in order to determine the susceptibility of 36 clinical isolates of E. corrodens to eight antimicrobial agents. MICs obtained by the HTM method yielded 95.5 and 84% agreement (within 2 and 1 log 2 dilutions, respectively) with those obtained by AD. The HTM method with incubation in CO 2 for 48 h was highly reproducible and constitutes an easy alternative for antimicrobial susceptibility testing of E. corrodens .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference14 articles.

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