The yggH Gene of Escherichia coli Encodes a tRNA (m 7 G46) Methyltransferase-Reference-Cited by-同舟云学术

The yggH Gene of Escherichia coli Encodes a tRNA (m 7 G46) Methyltransferase

Published:2003-05-15 Issue:10 Volume:185 Page:3238-3243
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

De Bie Lara G. S.¹,Roovers Martine¹,Oudjama Yamina²,Wattiez Ruddy³,Tricot Catherine²,Stalon Victor¹²,Droogmans Louis¹,Bujnicki Janusz M.⁴

Affiliation:

1. Laboratoire de Microbiologie, Université Libre de Bruxelles

2. Institut de Recherches Microbiologiques Jean-Marie Wiame, B-1070 Brussels

3. Laboratoire de Chimie Biologique, Université de Mons-Hainaut, B-7000 Mons, Belgium

4. Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland

Abstract

ABSTRACT We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S -adenosyl- l -methionine-dependent formation of N 7 -methylguanosine at position 46 (m 7 G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m 7 G46) methyltransferase activity.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.10.3238-3243.2003

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