Antibody-Targeted Lethal Photosensitization of Porphyromonas gingivalis

Author:

Bhatti M.12,MacRobert A.3,Henderson B.2,Shepherd P.4,Cridland J.4,Wilson M.1

Affiliation:

1. Department of Microbiology,1 and

2. Cellular Microbiology Research Group,2 Eastman Dental Institute, and

3. Department of Surgery, National Medical Laser Centre,3 University College London, and

4. Department of Immunobiology, GKT School of Medicine, King's College,4 London, United Kingdom

Abstract

ABSTRACT We have previously demonstrated that Porphyromonas gingivalis is susceptible to killing by toluidine blue O (TBO) when irradiated with light from a helium-neon (HeNe) laser. The aim of this study was to determine whether a TBO-antibody conjugate (Ab-TBO) could be used to specifically target P. gingivalis to lethal photosensitization in the presence of Streptococcus sanguis or human gingival fibroblasts (HGFs). When a mixture of P. gingivalis and S. sanguis was exposed to 4 μg of TBO/ml and irradiated with HeNe laser light, there were 1.5- and 4.0-log 10 -unit reductions in the viable counts, respectively. In contrast, when TBO was conjugated with a murine monoclonal antibody against P. gingivalis lipopolysaccharide, the reductions in viable counts of P. gingivalis and S. sanguis amounted to 5.0 and 0.1 log 10 units, respectively. Lethal photosensitization of P. gingivalis in the presence of HGFs using unconjugated TBO resulted in a 0.7-log 10 -unit reduction in P. gingivalis viable counts and a 99% reduction in the incorporation of tritiated thymidine ([ 3 H]Tdr) by the HGFs. In contrast, when the Ab-TBO conjugate was used, there was a 100% reduction in P. gingivalis viable counts but no significant reduction in the incorporation of [ 3 H]Tdr by HGFs. These results demonstrate that specific targeting of P. gingivalis can be achieved using TBO conjugated to a monoclonal antibody raised against a cell surface component of this organism.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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