Development of a Transposon Mutagenesis System in the Oral Spirochete Treponema denticola-Reference-Cited by-同舟云学术

Development of a Transposon Mutagenesis System in the Oral Spirochete Treponema denticola

Published:2008-10-15 Issue:20 Volume:74 Page:6461-6464
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yang Yu¹,Stewart Philip E.²,Shi Xiaoguang¹,Li Chunhao¹

Affiliation:

1. Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214

2. Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

Abstract

ABSTRACT Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola . A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola , generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01424-08

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