Effect of Substrate on the Fatty Acid Composition of Hydrocarbon- and Ketone-utilizing Microorganisms

Author:

Dunlap K. R.1,Perry J. J.1

Affiliation:

1. Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27607

Abstract

The fatty acid pattern in hydrocarbon- and ketone-utilizing bacteria after growth on various substrates was examined. The fatty acid composition of one hydrocarbon-utilizing organism ( Mycobacterium sp. strain OFS) was investigated in detail after growth on n -alkanes, 1-alkenes, ketones, and n -alcohols. n -Alkanes shorter than C 13 or longer than C 17 were not incorporated into cellular fatty acids without some degradation. Strain OFS incorporated C 14 to C 17 1-alkenes into cellular fatty acids as the ω-monoenoic fatty acid. Methyl ketones were incorporated into strain OFS after removal of one- or two-carbon fragments from the carbonyl end of the molecule. An organism isolated by enrichment on methyl ketones was incapable of n -alkane utilization but could grow on, although not incorporate, ketones or long chain n -alcohols into cellular fatty acids.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference9 articles.

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3. Hydrocarbons as substrates for microorganisms. Antonie van Leeuwenhoek J;Foster J. W.;Microbiol. Serol.,1962

4. Characterization of unsaturated fatty acids by gas-liquid chromatography;Hofstetter H. H.;J. Am. Oil Chemists' Soc.,1965

5. The oxidation of a-olefins by a Pseudomonas. Reactions involving the double bond. Antonie van Leeuwenhoek J;Huybregtse R.;Microbiol. Serol.,1944

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