Affiliation:
1. Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27607
Abstract
The fatty acid pattern in hydrocarbon- and ketone-utilizing bacteria after growth on various substrates was examined. The fatty acid composition of one hydrocarbon-utilizing organism (
Mycobacterium
sp. strain OFS) was investigated in detail after growth on
n
-alkanes, 1-alkenes, ketones, and
n
-alcohols.
n
-Alkanes shorter than C
13
or longer than C
17
were not incorporated into cellular fatty acids without some degradation. Strain OFS incorporated C
14
to C
17
1-alkenes into cellular fatty acids as the ω-monoenoic fatty acid. Methyl ketones were incorporated into strain OFS after removal of one- or two-carbon fragments from the carbonyl end of the molecule. An organism isolated by enrichment on methyl ketones was incapable of
n
-alkane utilization but could grow on, although not incorporate, ketones or long chain
n
-alcohols into cellular fatty acids.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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