Affiliation:
1. Department of Microbiology, California State College, Long Beach, California 90804
Abstract
Catabolic capabilities of
Leucothrix mucor
were studied by radiorespirometric technique with a liquid scintillation spectrometer. Through measurement of relative rates and total percentages of
14
CO
2
produced, together with the determination of the cellular incorporation of
14
C from different carbon atoms of various labeled substrates, such as simple carbohydrates, intermediary metabolites, and amino acids, the essential characteristics of the primary and secondary pathways operative in this organism were examined. These substrates appeared to be degraded mainly via enzymes of the Entner-Doudoroff pathway in conjunction with the tricarboxylic acid cycle. Estimation of concurrent participation of pathways indicated that 20 to 25% of the administered carbohydrate was catabolized via the pentose phosphate pathway. There was no evidence of the Embden-Meyerhof-Parnas pathway operating in this organism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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