Characterization of Escherichia coli strains with gapA and gapB genes deleted-Reference-Cited by-同舟云学术

Characterization of Escherichia coli strains with gapA and gapB genes deleted

Published:1997-08 Issue:16 Volume:179 Page:5218-5221
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Seta F D¹,Boschi-Muller S¹,Vignais M L¹,Branlant G¹

Affiliation:

1. Laboratoire d'Enzymologie et de Génie Génétique, Université Henri Poincaré Nancy I, URA CNRS 457, Vandoeuvre-les-Nancy, France.

Abstract

We obtained a series of Escherichia coli strains in which gapA, gapB, or both had been deleted. Delta gapA strains do not revert on glucose, while delta gapB strains grow on glycerol or glucose. We showed that gapB-encoded protein is expressed but at a very low level. Together, these results confirm the essential role for gapA in glycolysis and show that gapB is dispensable for both glycolysis and the pyridoxal biosynthesis pathway.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.179.16.5218-5221.1997

Reference23 articles.

1. Identification, molecular cloning and sequence analysis of a gene cluster encoding the Class II fructose 1,6 bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli;Alefounder P. R.;Mol. Microbiol.,1989

2. Identification of the 37-kDa protein displaying a variable interaction with the erythroid cell membrane as glyceraldehyde-3-phosphate dehydrogenase;Allen R. W.;J. Biol. Chem.,1987

3. Linkage map of Escherichia coli K-12, edition 7;Bachmann B. J.;Microbiol. Rev.,1983

4. Comparative enzymatic properties of gapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3- phosphate dehydrogenase;Boschi-Muller S.;J. Biol. Chem.,1997

5. Boschi-Muller S. and G. Branlant. Unpublished results.

Cited by 43 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The gap gene of Rhizobium etli is required for both free life and symbiosis with common beans.;Microbiological Research;2024-07

2. Protein engineering and iterative multimodule optimization for vitamin B6 production in Escherichia coli;Nature Communications;2023-08-31

3. Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration;Biotechnology Advances;2023-03

4. CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids;Nucleic Acids Research;2022-12-09

5. Engineering of Escherichia coli Glyceraldehyde-3-Phosphate Dehydrogenase with Dual NAD+/NADP+ Cofactor Specificity for Improving Amino Acid Production;Microorganisms;2022-05-06