Affiliation:
1. Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA.
Abstract
The cspD gene of Escherichia coli encodes a protein of high sequence similarity with the cold shock protein CspA, but cspD expression is not induced by cold shock. In this study, we analyzed the regulation of cspD gene expression. By using a cspD-lacZ fusion and primer extension analysis, the expression of cspD was found to be dramatically induced by stationary-phase growth. However, this induction does not depend on the stationary-phase sigma factor sigmaS. Moreover, the expression of cspD is inversely dependent on growth rates and induced upon glucose starvation. Using a (p)ppGpp-depleted strain, we found that (p)ppGpp is one of the positive factors for the regulation of cspD expression.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference39 articles.
1. Evidence for two functional gal promoter in intact Escherichia coli cells;Aiba H.;J. Biol. Chem.,1981
2. Division genes in Escherichia coli are expressed coordinately to cell septum requirements by gearbox promoters;Aldea M.;EMBO J.,1990
3. Basic local alignment search tool;Altshul S. F.;J. Mol. Biol.,1990
4. Bae W. and M. Inouye. Unpublished data.
5. Berlyn M. K. B. K. B. Low K. E. Rudd and M. Singer. 1996. Linkage map of Escherichia coli K-12 edition 9 p. 1715-1902. In F. C. Neidhardt R. Curtiss III J. L. Ingraham E. C. C. Lin K. B. Low B. Magasanik W. S. Reznikoff M. Riley M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella: cellular and molecular biology 2nd ed. ASM Press Washington D.C.
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