Cetyl-Pyridinium Chloride Is Useful for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage

Author:

Pardini Manuela1,Varaine Francis2,Iona Elisabetta1,Arzumanian Erchanik3,Checchi Francesco4,Oggioni Marco Rinaldo5,Orefici Graziella1,Fattorini Lanfranco1

Affiliation:

1. Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome

2. Médecins Sans Frontières

3. Guliripchi Tuberculosis Hospital, Sukhumi, Abkhazia

4. Epicentre, Paris, France

5. Dipartimento di Biologia Molecolare, Università di Siena, Siena, Italy

Abstract

ABSTRACT Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 ± 9 days was significantly higher than that from sputa that were untreated and processed by the N -acetyl- l -cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

1. Comparison of Sodium Carbonate, Cetyl-Pyridinium Chloride, and Sodium Borate for Preservation of Sputa for Culture of Mycobacterium tuberculosis

2. Master R. N. 1992. Mycobacteriology p. 3.0.1-3.1.6.4. In H. D. Isenberg (ed.) Clinical microbiology procedures handbook. American Society for Microbiology Washington D.C.

3. Metchock B. G. F. S. Nolte and R. J. Wallace Jr. 1999. Mycobacterium p. 399-437. In P. R. Murray E. J. Baron M. A. Pfaller F. C. Tenover and R. H. Yolken (ed.) Manual of clinical microbiology. American Society for Microbiology Washington D.C.

4. Effect of cetylpyridinium chloride on pathogenic fungi and Nocardia asteroides in sputum

5. Ramarokoto, H., H. Randriamiharisoa, A. Rakotoarisaonina, T. Rasolovavalona, V. Rasolofo, S. Chanteau, M. Ralamboson, B. Cauchoix, and D. Rakotondramarina. 2002. Bacteriological follow-up of tuberculosis treatment: a comparative study of smear microscopy and culture results at the second month of treatment. Int. J. Tuberc. Lung Dis.6:909-912.

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