Affiliation:
1. Environmental Simulation and Pollution Control State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People’s Republic of China
Abstract
ABSTRACT
Antialgal allelochemicals were isolated from
Phragmites communis
Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of
Chlorella pyrenoidosa
and
Microcystis aeruginosa
but had no inhibition on
Chlorella vulgaris.
The 50% effective concentrations (EC
50
) of the allelopathic fractions on
C. pyrenoidosa
and
M. aeruginosa
were 0.49 and 0.79 mg/liter, respectively. The allelopathic activity of the fraction was species-specific. The isolated allelopathic fraction caused metal ion leakage from algal cells. The fraction decreased the activities of antioxidant enzymes, such as superoxide dismutase and peroxidase. The addition of the isolated fraction increased the concentration of unsaturated lipid fatty acids in cell membrane of
C. pyrenoidosa
and
M. aeruginosa
. This caused a change in plasma membrane integrity and the leakage of ions in the protoplast. The allelopathic compound was identified by nuclear magnetic resonance and gas chromatography-mass spectrometry as ethyl 2-methylacetoacetate. Synthesized ethyl 2-methylacetoacetate also showed allelopathic activity on
C. pyrenoidosa
and
M. aeruginosa
. The EC
50
of synthesized ethyl 2-methylacetoacetate on
C. pyrenoidosa
and
M. aeruginosa
were 0.49 and 0.65 mg/liter, respectively.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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