Detection of Carbapenemase Producers in Enterobacteriaceae by Use of a Novel Screening Medium

Author:

Nordmann Patrice1,Girlich Delphine1,Poirel Laurent1

Affiliation:

1. Service de Bactériologie-Virologie, INSERM U914 “Emerging Resistance to Antibiotics,” Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France

Abstract

ABSTRACT A Drigalski agar-based culture medium containing an ertapenem, cloxacillin, and zinc sulfate (Supercarba medium) was tested for screening carbapenemase-producing members of the family Enterobacteriaceae . OXA-48 ( n = 44), NDM ( n = 25), VIM or IMP ( n = 27), and KPC producers ( n = 18) were detected with a low detection limit. Its overall sensitivity (95.6%) was higher than those of the currently available ChromID ESBL (bioMérieux) and CHROMagar KPC (CHROMagar) screening media. The Supercarba medium provides a significant improvement for detection of the most common types of carbapenemase producers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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