Stable Cell Clones Harboring Self-Replicating SARS-CoV-2 RNAs for Drug Screen-Reference-Cited by-同舟云学术

Stable Cell Clones Harboring Self-Replicating SARS-CoV-2 RNAs for Drug Screen

Published:2022-03-23 Issue:6 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Liu Shufeng¹^ORCID,Chou Chao-Kai²,Wu Wells W.²,Luan Binquan³,Wang Tony T.¹^ORCID

Affiliation:

1. Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA

2. Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA

3. Computational Biological Center, IBM Thomas J. Watson Research Center, Yorktown Heights, New York, USA

Abstract

SARS-CoV-2 replicon systems that have been reported up to date were unsuccessful in deriving stable cell lines harboring non-cytopathic replicons. The transient expression of viral sgmRNA or a reporter gene makes it impractical for industry-scale screening of large compound libraries using these systems.

Funder

HHS | U.S. Food and Drug Administration

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.02216-21

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