Affiliation:
1. Laboratory of Dairying, University of British Columbia, Vancouver, British Columbia, Canada
Abstract
Gronlund, Audrey
F. (University of British Columbia, Vancouver, Canada)
and J. J. R. Campbell
. Nitrogenous substrates of endogenous respiration in
Pseudomonas aeruginosa
. J. Bacteriol.
86:
58–66. 1963.—The nature of the nitrogenous reserves of
Pseudomonas aeruginosa
that are oxidized during endogenous respiration was studied by following the changes in the chemical constituents and in the distribution of radioactivity of starving cells that had been grown on C
14
-labeled substrates. The total protein and nucleic acid of Warburg vessel contents decreased during starvation. Deoxyribonucleic acid increased slightly, whereas ribonucleic acid (RNA) decreased. C
14
O
2
was evolved from endogenously respiring cells specifically labeled in the nucleic acid fraction and from cells specifically labeled in the protein fraction. Chemical fractionation of C
14
-labeled cells showed a decrease in hot trichloroacetic acid-soluble and -insoluble compounds, indicating that the C
14
O
2
arose from the degradation of RNA and protein and not free pool compounds. A decrease in ribosomal RNA and protein was evident from physical fractionations of starved, labeled cells. An enzyme responsible for the initiation of ribosomal degradation was found to be associated with the ribosome fraction. It was concluded that oxidation of the ribonucleo-protein during endogenous respiration may be a general phenomenon in microorganisms.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference31 articles.
1. Nucleic acid metabolism in relation to the lysogenic phenomenon;BOREK E., A.;J. Bacteriol.,1955
2. A ribonucleoprotein component of Escherichia coli;BOWEN T. J.;Biochem. J.,1959
3. CONWAY E. J. 1950. Microdiffusion analysis and volumetric error 3rd ed. Crosby Lockwood and Sons Ltd. London.
4. Endogenous metabolism of;CAMPBELL J. J.;Pseudomonas. Ann. N. Y. Acad. Sci.,1963
5. Enzyme distribution in Pseudomonas aeruginosa;CAMPBELL J. J.;J. Bacteriol.,1962
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